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Vesicular GABA transporter (VGAT) Antibody

Applications

  • WB
  • IHC
  • ICC

Reactivity

  • Mouse
  • Rat
  • Chicken
  • Xenopus

Predicted Reactivity

  • Dog
  • Bovine
  • Non-Human Primate
Overview
Catalog # bs-70309R
Product Name Vesicular GABA transporter (VGAT) Antibody
Applications WB, IHC, ICC
Specificity Specific for endogenous levels of the ~53 kDa VGAT protein.
Reactivity Mouse, Rat, Chicken, Xenopus
Predicted Reactivity Dog, Bovine, Non-Human Primate
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat VGAT, conjugated to keyhole limpet hemocyanin (KLH).
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification Antigen Affinity purification from Pooled whole antiserum
Storage Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID 83612
Swiss Prot O35458
Synonyms bA122O1.1 antibody, GABA and glycine transporter antibody, hVIAAT antibody, SLC32A1 antibody, SLC32A 1 antibody, Slc32a1 antibody, solute carrier family 32 (GABA vesicular transporter) member 1 antibody, Solute carrier family 32 member 1 antibody, Vesicular GABA Amino Acid Transporter antibody, Vesicular GABA transporter antibody, Vesicular inhibitory amino acid transporter antibody, VGAT antibody, VIAAT antibody, VIAAT_HUMAN antibody
Background The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
Application Dilution
WB 1:300-5000
IHC
ICC 1:100-500