Argonaute 2 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70341r-100uL |
| Product Name | Argonaute 2 Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of Ago2 on SDS-PAGE immunoblots of human A431 cells and mouse recombinant Ago2 full length protein. |
| Reactivity | Human, Mouse, Rat, Chicken, Xenopus |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Ago2 synthetic peptide (coupled to KLH) corresponding to amino acids from the central region of human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q9UKV8 |
| Synonyms | Ago2, Argonaute, eIF-2C2, eIF2C2; protein slicer |
| Background | Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Argonaute 2 Antibody (bs-70341R) validated for, and at what dilution?
bs-70341R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Argonaute 2 Antibody (bs-70341R) react with?
Tested reactivity: Human, Mouse, Rat, Chicken, Xenopus.
How should bs-70341R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Argonaute 2 Antibody (bs-70341R)?
Ago2 synthetic peptide (coupled to KLH) corresponding to amino acids from the central region of human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2.