Argonaute 2 (Tyr-393), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70343r-100uL |
| Product Name | Argonaute 2 (Tyr-393), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | This antibody was cross-absorbed to unphospho-Ago2 (Tyr-393), before affinity purification using phospho-Ago2 (Tyr-393) peptide. The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of phosphorylated Ago2 on SDS-PAGE immunoblots of human A431 cells that were treated with EGF. |
| Reactivity | Human, Mouse, Rat, Chicken, Xenopus |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phospho-Ago2 (Tyr-393) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 393 in human Ago2. |
| Modification Site | Tyr-393 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q9UKV8 |
| Synonyms | Ago2, Argonaute, eIF-2C2, eIF2C2; protein slicer |
| Background | Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Argonaute 2 (Tyr-393), Phosphospecific Antibody (bs-70343R) validated for, and at what dilution?
bs-70343R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Argonaute 2 (Tyr-393), Phosphospecific Antibody (bs-70343R) react with?
Tested reactivity: Human, Mouse, Rat, Chicken, Xenopus.
How should bs-70343R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Argonaute 2 (Tyr-393), Phosphospecific Antibody (bs-70343R)?
Phospho-Ago2 (Tyr-393) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 393 in human Ago2.