Bad (Ser-112), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70358r-200uL |
| Product Name | Bad (Ser-112), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 23 kDa* doublet corresponding to the apparent molecular mass of phosphorylated Bad on SDS-PAGE immunoblots of mouse J774A.1 treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. This peptide sequence is highly conserved in human (Ser-75) and rat (Ser-113) Bad. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phospho-Bad (Ser-112) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 112 in mouse Bad. |
| Modification Site | Ser-112 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 0.05% NaN3 |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Swiss Prot | Q61337 |
| Synonyms | Bcl2 antagonist of cell death, BAD; Bcl-2-binding component 6, Bbc6, Bcl-xL/Bcl-2-associated death promoter |
| Background | Bad is a member of the BCL-2 family of regulators involved in programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT IKK, and MAP kinases, as well as protein phosphatase calcineurin are found to be involved in the regulation of this Bad activity. Phosphorylation of Bad occurs on one or more of Ser-26, Ser-112, Ser-136, and Ser-155 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-136 or Ser-112 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-155, a site within the BH3 motif, leading to the release of Bcl-xL and the promotion of cell survival. Ser-26 is phosphorylated by IKK leading to phosphorylation of C-terminal serine sites and disruption of binding to Bcl-xL. This inactivation of Bad inhibits TNFα-induced apoptosis independent of NF-κB activity. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Bad (Ser-112), Phosphospecific Antibody (bs-70358R) validated for, and at what dilution?
bs-70358R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bad (Ser-112), Phosphospecific Antibody (bs-70358R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-70358R be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: PBS + 0.05% NaN3
What immunogen was used to raise Bad (Ser-112), Phosphospecific Antibody (bs-70358R)?
Phospho-Bad (Ser-112) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 112 in mouse Bad.