| Overview |
| bs-70607R |
| Phosphothreonine Antibody |
| WB, IP, ICC |
| This antibody was cross-adsorbed to unphosphorylated peptide then Affinity purification using phospho-threonine peptide (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot and ELISA. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Rabbit |
| Phosphothreonine synthetic peptides coupled to carrier protein. |
| pThr |
| Polyclonal |
| IgG |
| Lot dependent |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| N/A |
| Phosphoser/thr mAb |
| Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity |
| Application Dilution |
| WB |
1:300-5000 |
| IP |
1-2ug |
| ICC |
1:100-500 |
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