Sox2 (Lys-119), methyl-specific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70671r-100uL |
| Product Name | Sox2 (Lys-119), methyl-specific Antibody |
| Applications |
WB
|
| Specificity | The antibody was cross adsorbed to unmethylated Sox2 (Lys-119) peptide before affinity purification using methylated-Sox2 (Lys-119) peptide. |
| Reactivity | Human, Mouse, Rat, Chicken, Xenopus |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Methylated-Sox2 (Lys-119) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Lys-119 in mouse Sox2. |
| Modification Site | Lys-119 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P48432 |
| Synonyms | SRY (sex determining region Y) box-2, ANOP3, MCOPS3, SOX-2 |
| Background | Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Sox2 (Lys-119), methyl-specific Antibody (bs-70671R) validated for, and at what dilution?
bs-70671R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Sox2 (Lys-119), methyl-specific Antibody (bs-70671R) react with?
Tested reactivity: Human, Mouse, Rat, Chicken, Xenopus.
How should bs-70671R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Sox2 (Lys-119), methyl-specific Antibody (bs-70671R)?
Methylated-Sox2 (Lys-119) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Lys-119 in mouse Sox2.