WWP1 (N-terminus) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70733r-100uL |
| Product Name | WWP1 (N-terminus) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 110 kDa* protein corresponding to the apparent molecular mass of WWP1 on SDS-PAGE immunoblots of adult mouse liver and human PC3 cells. The antibody also detects lower molecular weight isoforms of WWP1 in mouse liver, and human PC3, HepG2, K562, HeLa, and HUVEC. This sequence is highly conserved in rat and mouse WWP1, and has low homology to other NEDD4-like family members. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | WWP1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human WWP1. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q9H0M0 |
| Synonyms | AIP5,Tiul1; hSDRP1 |
| Background | The Nedd4-like family of E3 ubiquitin ligases have been implicated in several types of human cancer. There are nine members of the Nedd4-like E3 family, all of which have an N-terminal C2 domain, two to four WW domains in the central region, and a C-terminal domain that is homologous to the C-terminus of E6-AP. This ubiquitin ligase family includes NEDD4-1, NEDD4-2, AIP4/Itch, AIP5/WWP1, SMURF1 and SMURF2. Several ubiquitin ligases in this family are overexpressed in human cancers. WWP1 has been implicated as an oncogene as it is overexpressed in prostate and breast cancers, and knockdown of this ligase reduces cell proliferation and causes apoptosis. In addition, WWP1 has been shown to negatively regulate transforming growth factor-β (TGFβ) signaling by targeting TGFβ receptor 1, Smad2, and Smad4 for ubiquitin-mediated degradation. WWP1 has also been shown to regulate the protein levels of the epithelial sodium channel (ENaC), Notch, EGFR/ErbB2, Runx2, KLF2, KLF5, p53, and p63. Thus, WWP1 may regulate cell development and proliferation through the degradation of proteins involved in a variety of cell signaling pathways. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is WWP1 (N-terminus) Antibody (bs-70733R) validated for, and at what dilution?
bs-70733R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does WWP1 (N-terminus) Antibody (bs-70733R) react with?
Tested reactivity: Human, Mouse, Rat, Chicken.
How should bs-70733R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise WWP1 (N-terminus) Antibody (bs-70733R)?
WWP1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human WWP1.