5-Bromo-2'-deoxyuridine

Overview
Catalog #	bs-75964c-1g
Product Name	5-Bromo-2'-deoxyuridine
Specifications
Storage Buffer	Powder
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 59-14-3 Molecular formula: C9H11BrN2O5 Molecular weight: 307.1 Purity: >99% (HPLC) Appearance: White powder. Solubility: Soluble in DMSO (30mg/ml), DMF (30mg/ml) or ethanol (25mg/ml) or water (10mg/ml). InChiKey: WOVKYSAHUYNSMH-RRKCRQDMSA-N SMILES: O[C@H]1C[C@H](N2C=C(Br)C(NC2=O)=O)O[C@@H]1CO
Description	The synthetic nucleoside 5-Bromo-2'-deoxyuridine (BrdU) is a thymidine analog used to label DNA and commonly used in the detection of proliferating cells in living tissues. It can be incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies with fluorescent tags to target the BrdU incorporation for detection via flow cytometry or fluorescence microscopy. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat. BrdU is also used as a mutagen in genetic research. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. BrdU replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.