BAGE4 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9116R-Biotin-100uL |
| Product Name | BAGE4 Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BAGE4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 3624 |
| Synonyms | B melanoma antigen 4; B melanoma antigen family, member 4; BAGE4; BAGE4_HUMAN; Cancer/testis antigen 2.4; CT2.4; MGC119851; MGC119852; MGC119853; MLL3P. |
| Background | Members of the BAGE gene family encode antigens that are recognized by cytotoxic T lymphocytes and are also known as CT (cancer/testis) antigens. Generated by juxtacentromeric shuffling of the MLL3 gene, the ancestral BAGE gene was expanded by acrocentric exchanges and/or juxtacentromeric movements. BAGE, also known as B melanoma antigen 1 or cancer/testis antigen 2.1, is a 43 amino acid member of the BAGE family. Generally, BAGE proteins are not expressed in normal tissues, except in testis, but are expressed highly in melanomas, bladder carcinomas, head and neck squamous cell carcinomas, and lung and breast cancer carcinomas. BAGE proteins are not expressed in renal, colorectal and prostatic carcinomas, leukemias and lymphomas. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | |
Frequently asked questions
Which applications is BAGE4 Polyclonal Antibody, Biotin Conjugated (bs-9116R-Biotin) validated for, and at what dilution?
bs-9116R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BAGE4 Polyclonal Antibody, Biotin Conjugated (bs-9116R-Biotin) react with?
Tested reactivity: Human.
How should bs-9116R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BAGE4 Polyclonal Antibody, Biotin Conjugated (bs-9116R-Biotin)?
KLH conjugated synthetic peptide derived from human BAGE4