fbpB Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-9268R-100uL |
| Product Name | fbpB Polyclonal Antibody |
| Applications |
WB
IHC-P
IF(IHC-P)
|
| Specificity | This antibody will recognize Mycobacterium tuberculosis Ag85A and Ag85B |
| Reactivity | Bacteria |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Ag85B |
| Immunogen Range | 231-325/325 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 885785, 886132 |
| Swiss Prot | P9WQP1, P9WQP3 |
| Subcellular location | Secreted |
| Synonyms | Antigen 85 complex B; 30 kDa extracellular protein; Mycobacterium; Mycolyl transferase 85B; fbpB; Antigen 85-B; 85B; Ag85B; Extracellular alpha-antigen; Fibronectin-binding protein B; DGAT, Acyl-CoA:diacylglycerol acyltransferase, Ag85A, fbpA, 85A |
| Background | The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is fbpB Polyclonal Antibody (bs-9268R) validated for, and at what dilution?
bs-9268R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does fbpB Polyclonal Antibody (bs-9268R) react with?
Tested reactivity: Bacteria.
How should bs-9268R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise fbpB Polyclonal Antibody (bs-9268R)?
KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Ag85B Immunogen range: 231-325/325.