CD5 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33077m-100uL |
| Product Name | CD5 Monoclonal Antibody |
| Applications |
WB
IHC-F
IF(IHC-P)
IHC-P
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human CD5 |
| Clonality | Monoclonal |
| Clone # | 12A8 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 921 |
| Swiss Prot | P06127 |
| Synonyms | T-cell surface glycoprotein CD5; Lymphocyte antigen 1; Ly-1; Lyt-1; CD-5; CD5 antigen; CD 5; CD5 molecule; CD5 antigen (p56 62); CD5_HUMAN; LEU 1; LEU1; Ly12; LyA; Lymphocyte Antigen CD5; Lymphocyte antigen T1/Leu 1; Lymphocyte antigen T1/Leu-1; Lymphocyte glycoprotein T1/Leu1; OTTHUMP00000236973; p56 62; T1. |
| Background | This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-F | 1:50-200 |
| IF(IHC-P) | 1:50-200 |
| IHC-P | 1:50-200 |
| FCM | 10ul/10^6 cells in 100ul |
Frequently asked questions
Which applications is CD5 Monoclonal Antibody (bsm-33077M) validated for, and at what dilution?
bsm-33077M is validated for WB (1:300-5000); IHC-F (1:50-200); IF(IHC-P) (1:50-200); IHC-P (1:50-200); FCM (10ul/10^6 cells in 100ul). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD5 Monoclonal Antibody (bsm-33077M) react with?
Tested reactivity: Human.
How should bsm-33077M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD5 Monoclonal Antibody (bsm-33077M)?
KLH conjugated synthetic peptide derived from human CD5