Histone H3 (tri methyl K4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33110m-100uL |
| Product Name | Histone H3 (tri methyl K4) Monoclonal Antibody |
| Applications |
WB
ELISA
IHC-F
IF(ICC)
IHC-P
|
| Reactivity | Human, Mouse, Rat, Others |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Histone H3 (tri methyl K4) |
| Modification Site | K4 |
| Clonality | Monoclonal |
| Clone # | 4E11 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 8290 |
| Swiss Prot | P68431 |
| Synonyms | Histone Cluster 3, H3; H3 Histone Family, Member T; Histone 3, H3; H3FT; H3/G; H3/T; H3t; H3.4 ; Histone H3.1t ; HIST3H3; HGNC:4778; H31T_HUMAN |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Histone H3 (tri methyl K4) Monoclonal Antibody (bsm-33110M) validated for, and at what dilution?
bsm-33110M is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500); IF(ICC) (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (tri methyl K4) Monoclonal Antibody (bsm-33110M) react with?
Tested reactivity: Human, Mouse, Rat, Others.
How should bsm-33110M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (tri methyl K4) Monoclonal Antibody (bsm-33110M)?
KLH conjugated synthetic peptide derived from human Histone H3 (tri methyl K4)