Histone H3 (di methyl K9) Monoclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-33114m-pe-cy5-100uL |
| Product Name | Histone H3 (di methyl K9) Monoclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Histone H3(di methyl K9) |
| Modification Site | K9 |
| Clonality | Monoclonal |
| Clone # | 5D2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8290 |
| Swiss Prot | P68431 |
| Synonyms | Histone Cluster 3, H3; H3 Histone Family, Member T; Histone 3, H3; H3FT; H3/G; H3/T; H3t; H3.4; Histone H3.1t; HIST3H3; HGNC:4778; H31T_HUMAN |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Histone H3 (di methyl K9) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33114M-PE-Cy5) validated for, and at what dilution?
bsm-33114M-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (di methyl K9) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33114M-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow.
How should bsm-33114M-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (di methyl K9) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33114M-PE-Cy5)?
KLH conjugated synthetic peptide derived from human Histone H3(di methyl K9)
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-33114M-PE-Cy5)?
PE-Cy5 on bsm-33114M-PE-Cy5: excitation / emission 488nm/670nm.