ACTH Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (Human pituitary adenoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACTH ) Monoclonal Antibody, Unconjugated (bsm-34147M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human pituitary adenoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACTH ) Monoclonal Antibody, Unconjugated (bsm-34147M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-34147m
Product Name	ACTH Monoclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human ACTH
Clonality	Monoclonal
Clone #	7D13
Isotype	IgG1/Kappa
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5443
Swiss Prot	P01189
Subcellular location	Secreted
Synonyms	Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; Alpha Melanocyte Stimulating Hormone; Beta Lipotropin; Beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin Beta; Lipotropin Gamma; LPH; Melanotropin Alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin; COLI_HUMAN.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF