SARS-CoV-2 (2019-nCoV) Spike Neutralizing Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-41636M-100uL |
| Product Name | SARS-CoV-2 (2019-nCoV) Spike Neutralizing Monoclonal Antibody |
| Applications |
ELISA
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant SARS-CoV-2 Spike S1 Protein |
| Immunogen Range | 14-685/1213 |
| Clonality | Monoclonal |
| Clone # | 7H3 |
| Isotype | IgG3 |
| Concentration | Lot Dependent |
| Purification | affinity purified by Protein A |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 43740568 |
| Swiss Prot | P0DTC2 |
| Synonyms | SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2. |
| Background | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| Application Dilution | |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is SARS-CoV-2 (2019-nCoV) Spike Neutralizing Monoclonal Antibody (bsm-41636M) validated for, and at what dilution?
bsm-41636M is validated for ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SARS-CoV-2 (2019-nCoV) Spike Neutralizing Monoclonal Antibody (bsm-41636M) react with?
Tested reactivity: Virus.
How should bsm-41636M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SARS-CoV-2 (2019-nCoV) Spike Neutralizing Monoclonal Antibody (bsm-41636M)?
Recombinant SARS-CoV-2 Spike S1 Protein Immunogen range: 14-685/1213.