ALDH1A1 (2C2) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51032M-100uL |
| Product Name | ALDH1A1 (2C2) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein. |
| Clonality | Monoclonal |
| Clone # | 2C2 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 216 |
| Swiss Prot | P00352 |
| Subcellular location | Cytoplasm |
| Synonyms | ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e; Retinal dehydrogenase 1; RALDH 1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; ALDH1A1. |
| Background | ALDH1A1 belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. ALDH1A1 is the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. ALDH1A1 binds free retinal and cellular retinol-binding protein-bound retinal. ALDH1A1 can convert/oxidize retinaldehyde to retinoic acid. ALDH1A1 may have a broader specificity and oxidize other aldehydes in vivo. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:200-400 |
Frequently asked questions
Which applications is ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) validated for, and at what dilution?
bsm-51032M is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400); FCM (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) react with?
Tested reactivity: Human.
How should bsm-51032M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M)?
This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein.