RET (8C3) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51046M-100uL |
| Product Name | RET (8C3) Monoclonal Antibody |
| Applications |
WB
IF
IHC-P
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This RET Monoclonal antibody was raised using purified His-tagged recombinant human RET. |
| Clonality | Monoclonal |
| Clone # | 8C3 |
| Isotype | IgM |
| Concentration | Lot Dependent |
| Purification | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5979 |
| Swiss Prot | P07949 |
| Synonyms | PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1; Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret; RET |
| Background | Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is RET (8C3) Monoclonal Antibody (bsm-51046M) validated for, and at what dilution?
bsm-51046M is validated for WB (1:300-5000); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RET (8C3) Monoclonal Antibody (bsm-51046M) react with?
Tested reactivity: Human, Mouse.
How should bsm-51046M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RET (8C3) Monoclonal Antibody (bsm-51046M)?
This RET Monoclonal antibody was raised using purified His-tagged recombinant human RET.