C-rel (8C2) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51087M-100uL |
| Product Name | C-rel (8C2) Monoclonal Antibody |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This REL monoclonal antibody is generated from mouse immunized with REL recombinant protein. |
| Clonality | Monoclonal |
| Clone # | 8C2 |
| Isotype | IgG2b |
| Concentration | Lot Dependent |
| Purification | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5966 |
| Swiss Prot | Q04864 |
| Synonyms | C-Rel; Proto-oncogene c-Rel; REL |
| Background | Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is C-rel (8C2) Monoclonal Antibody (bsm-51087M) validated for, and at what dilution?
bsm-51087M is validated for WB (1:300-5000); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does C-rel (8C2) Monoclonal Antibody (bsm-51087M) react with?
Tested reactivity: Human.
How should bsm-51087M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C-rel (8C2) Monoclonal Antibody (bsm-51087M)?
This REL monoclonal antibody is generated from mouse immunized with REL recombinant protein.