BCL2L1/Bcl-xL (4C4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51229M-100uL |
| Product Name | BCL2L1/Bcl-xL (4C4) Monoclonal Antibody |
| Applications |
WB
ELISA
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Purified His-tagged BCL2L1 protein was used to produced this monoclonal antibody. |
| Clonality | Monoclonal |
| Clone # | 4C4 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 598 |
| Swiss Prot | Q07817 |
| Synonyms | BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S; Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1 |
| Background | Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). Isoform Bcl-X(S) promotes apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is BCL2L1/Bcl-xL (4C4) Monoclonal Antibody (bsm-51229M) validated for, and at what dilution?
bsm-51229M is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BCL2L1/Bcl-xL (4C4) Monoclonal Antibody (bsm-51229M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-51229M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BCL2L1/Bcl-xL (4C4) Monoclonal Antibody (bsm-51229M)?
Purified His-tagged BCL2L1 protein was used to produced this monoclonal antibody.