ATG4A (14C9) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51292M-100uL |
| Product Name | ATG4A (14C9) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IF
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This ATG4A antibody is generated from a mouse immunized with a recombinant protein. |
| Clonality | Monoclonal |
| Clone # | 14C9 |
| Isotype | IgG2b |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 115201 |
| Swiss Prot | Q8WYN0 |
| Synonyms | APG4A; AUTL2; Cysteine protease ATG4A; AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A; ATG4A |
| Background | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is ATG4A (14C9) Monoclonal Antibody (bsm-51292M) validated for, and at what dilution?
bsm-51292M is validated for WB (1:300-5000); IF(ICC) (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATG4A (14C9) Monoclonal Antibody (bsm-51292M) react with?
Tested reactivity: Human.
How should bsm-51292M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATG4A (14C9) Monoclonal Antibody (bsm-51292M)?
This ATG4A antibody is generated from a mouse immunized with a recombinant protein.