YES1 (2C5) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51340M-100uL |
| Product Name | YES1 (2C5) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This YES1 antibody is generated from a mouse immunized with a recombinant protein of human YES1. |
| Clonality | Monoclonal |
| Clone # | 2C5 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 7525 |
| Swiss Prot | P07947 |
| Synonyms | Yes; c-yes; HsT441; P61-YES; Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; YES1 |
| Background | Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is YES1 (2C5) Monoclonal Antibody (bsm-51340M) validated for, and at what dilution?
bsm-51340M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does YES1 (2C5) Monoclonal Antibody (bsm-51340M) react with?
Tested reactivity: Human.
How should bsm-51340M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise YES1 (2C5) Monoclonal Antibody (bsm-51340M)?
This YES1 antibody is generated from a mouse immunized with a recombinant protein of human YES1.