APEX1 (15C2) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51448M-100uL |
| Product Name | APEX1 (15C2) Monoclonal Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This APEX1 antibody is generated from a mouse immunized with a recombinant protein of human APEX1. |
| Clonality | Monoclonal |
| Clone # | 15C2 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 328 |
| Swiss Prot | P27695 |
| Synonyms | APE; APX; APE1; APEN; APEX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1; APEX1 |
| Background | Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is APEX1 (15C2) Monoclonal Antibody (bsm-51448M) validated for, and at what dilution?
bsm-51448M is validated for WB (1:500-2000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APEX1 (15C2) Monoclonal Antibody (bsm-51448M) react with?
Tested reactivity: Human, Rat.
How should bsm-51448M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APEX1 (15C2) Monoclonal Antibody (bsm-51448M)?
This APEX1 antibody is generated from a mouse immunized with a recombinant protein of human APEX1.