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CALCA Monoclonal Antibody

The TT (treated with BFA ) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CALCA antibody (bsm-51512M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
The TT (treated with BFA ) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CALCA antibody (bsm-51512M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
25 ug total protein per lane of various lysates (see on figure) probed with CALCA monoclonal antibody, unconjugated (bsm-51512M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with CALCA monoclonal antibody, unconjugated (bsm-51512M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-51512m-100uL
Product Name CALCA Monoclonal Antibody
Applications
FCM
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human CALCA
Immunogen Range 51-130/141
Clonality Monoclonal
Clone # G8D24
Isotype IgM
Concentration Lot Dependent
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 796
Swiss Prot P01258
Subcellular location Secreted
Synonyms Katacalcin; Calcitonin; Alpha CGRP; CALC 1; CALC A; CALC1; Calcitonin related polypeptide alpha; CGRP 1; CGRP; CGRP I; CGRP-1; CGRPI; CGRP1; CT; KC; MGC126648; alpha type CGRP; calcitonin 1; calcitonin gene related peptide 1; calcitonin gene related peptide I; calcitonin isoform CT preproprotein; CALC_HUMAN.
Background Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Katacalcin is a potent plasma calcium-lowering peptide
Application Dilution
FCM 1:50-100

Frequently asked questions

Which applications is CALCA Monoclonal Antibody (bsm-51512M) validated for, and at what dilution?

bsm-51512M is validated for FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CALCA Monoclonal Antibody (bsm-51512M) react with?

Tested reactivity: Human.

How should bsm-51512M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CALCA Monoclonal Antibody (bsm-51512M)?

KLH conjugated synthetic peptide derived from human CALCA Immunogen range: 51-130/141.

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