ZAP70 Monoclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bsm-51603m-100uL |
| Product Name | ZAP70 Monoclonal Antibody |
| Applications |
WB
FLOW-CYT
ICC/IF
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human ZAP70. |
| Clonality | Monoclonal |
| Clone # | V4R3 |
| Concentration | Lot Dependent |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7535 |
| Swiss Prot | P43403 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ZAP-70; ZAP 70; zeta-associated protein 70; ZAP-70=protein tyrosine kinase Syk homolog {SH2-like and C-terminal kinase domains}; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; Syk-related tyrosine kinase. SRK; STD; TZK; ZAP70_HUMAN; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein. |
| Background | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR). |
| Application Dilution | |
| WB | =1:500-2000 |
| Flow-Cyt | Flow-Cyt=1:25 |
| ICC/IF | ICC/IF=1:25 |
Frequently asked questions
Which applications is ZAP70 Monoclonal Antibody (bsm-51603M) validated for, and at what dilution?
bsm-51603M is validated for WB (WB=1:500-2000); Flow-Cyt (Flow-Cyt=1:25); ICC/IF (ICC/IF=1:25). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-51603M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZAP70 Monoclonal Antibody (bsm-51603M)?
Recombinant human ZAP70.