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Ighg | Mouse IgG2a (Isotype Control) Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; \r\n(left panel)The section was incubated with CD44 Monoclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.\r\n(middle panel)The section was incubated with Ighg | Mouse IgG2a (Isotype Control) Monoclonal Antibody, Unconjugated (bsm-51772M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.\r\n(right panel)The section was incubated with PBS instead of the primary antibody overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; \r\n(left panel)The section was incubated with CD44 Monoclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.\r\n(middle panel)The section was incubated with Ighg | Mouse IgG2a (Isotype Control) Monoclonal Antibody, Unconjugated (bsm-51772M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.\r\n(right panel)The section was incubated with PBS instead of the primary antibody overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; \r\n(left panel)The section was incubated with CD44 Monoclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.\r\n(middle panel)The section was incubated with Ighg | Mouse IgG2a (Isotype Control) Monoclonal Antibody, Unconjugated (bsm-51772M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.\r\n(right panel)The section was incubated with PBS instead of the primary antibody overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; \r\n(left panel)The section was incubated with CD44 Monoclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.\r\n(middle panel)The section was incubated with Ighg | Mouse IgG2a (Isotype Control) Monoclonal Antibody, Unconjugated (bsm-51772M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.\r\n(right panel)The section was incubated with PBS instead of the primary antibody overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P
  • Flow-Cyt
  • ICC/IF
Overview
Catalog # bsm-51772m-100ul
Product Name Ighg | Mouse IgG2a (Isotype Control) Monoclonal Antibody
Applications
WB IHC-F IF IHC-P FLOW-CYT ICC/IF
Specifications
Conjugation Unconjugated
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Application Dilution
WB 1:1000-10000
IHC-F 1:200-1000
IF 1:200-1000
IHC-P 1:200-1000
Flow-Cyt 1:100-200
ICC/IF 1:100-500

Frequently asked questions

Which applications is Ighg | Mouse IgG2a (Isotype Control) Monoclonal Antibody (bsm-51772M) validated for, and at what dilution?

bsm-51772M is validated for WB (1:1000-10000); IHC-F (1:200-1000); IF (1:200-1000); IHC-P (1:200-1000); Flow-Cyt (1:100-200); ICC/IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-51772M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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