AKT2 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52011R-100uL |
| Product Name | AKT2 Recombinant Antibody |
| Applications |
WB
IF(ICC)
IF
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Synthetic peptide derived from human Akt2, around N-terminal. |
| Clonality | Monoclonal |
| Clone # | 2A1 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 208 |
| Swiss Prot | P31751 |
| Synonyms | C AKT; MGC9965; Oncogene AKT1; PKB; PRKBA; Protein Kinase B Alpha; RAC Alpha; RAC; RAC PK Alpha; RAC Serine/Threonine Protein Kinase; vAKT Murine Thymoma Viral Oncogene Homolog 1. |
| Background | IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. [subcellular location] Cytoplasmic and membrane-associated after cell stimulation leading to its translocation. In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. Belongs to the Ser/Thr protein kinase family. RAC subfamily. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is AKT2 Recombinant Antibody (bsm-52011R) validated for, and at what dilution?
bsm-52011R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AKT2 Recombinant Antibody (bsm-52011R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52011R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AKT2 Recombinant Antibody (bsm-52011R)?
Synthetic peptide derived from human Akt2, around N-terminal.