Histone H4 (tri methyl K20) Monoclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52112r-pe-cy55-100ul |
| Product Name | Histone H4 (tri methyl K20) Monoclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | Synthetic peptide derived from human Histone H4 (tri methyl K20), around 1-30aa (tri methyl K20). |
| Modification Site | tri methyl K20 |
| Clonality | Monoclonal |
| Clone # | 2C12 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 121504 |
| Swiss Prot | P62805 |
| Synonyms | Histone H4 Acetyl K12; Histone H4 Acetyl Lys12; Acetyl-Histone H4 Lys12; H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Histone H4 (tri methyl K20) Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-52112R-PE-CY5-5) validated for, and at what dilution?
BSM-52112R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H4 (tri methyl K20) Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-52112R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse.
How should BSM-52112R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H4 (tri methyl K20) Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-52112R-PE-CY5-5)?
Synthetic peptide derived from human Histone H4 (tri methyl K20), around 1-30aa (tri methyl K20).
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-52112R-PE-CY5-5)?
PE-Cy5.5 on BSM-52112R-PE-CY5-5: excitation / emission 488nm/694nm.