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phospho-STAT1 (Ser727) Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Huvec cell lysates probed with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human A549 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Huvec cell lysates probed with STAT1(S727) (8A1) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT1 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52209R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52209r-100uL
Product Name phospho-STAT1 (Ser727) Recombinant Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human STAT1 around the phosphorylation site of Ser727
Modification Site S727
Clonality Recombinant
Clone # 8A1
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6772
Swiss Prot P42224
Subcellular location Cytoplasm, Nucleus
Synonyms CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; 2010005J02Rik; DD6G4-4; STAT1_HUMAN; STAT1; Transcription factor ISGF-3 components p91/p84; STAT1_MOUSE; signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; STAT1 (phospho-S727); p-STAT1; phospho-STAT1; STAT1 (phospho-Ser727)
Background Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Application Dilution
WB IHC-P1:50-200
IHC-P 1:200-400
IHC-F 1:100-500
IF

Frequently asked questions

Which applications is phospho-STAT1 (Ser727) Recombinant Antibody (bsm-52209R) validated for, and at what dilution?

bsm-52209R is validated for WB(IHC-P) (1:50-200); IHC-P (1:200-400); IHC-F (1:100-500); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-STAT1 (Ser727) Recombinant Antibody (bsm-52209R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52209R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-STAT1 (Ser727) Recombinant Antibody (bsm-52209R)?

KLH conjugated Synthesised phosphopeptide derived from human STAT1 around the phosphorylation site of Ser727

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