STAT6 (9A7) Monoclonal Antibody

$Lane 1: NIH\/3T3 lysates; Lane 2: Hela lysates probed with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.$

Lane 1: NIH\/3T3 lysates; Lane 2: Hela lysates probed with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

$Hela cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.$

Hela cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

$HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.$

HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

$Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with STAT6 (9A7) Monoclonal Antibody at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with STAT6 (9A7) Monoclonal Antibody at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with STAT6 Antibody(bsm-52239R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with STAT6 Antibody(bsm-52239R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human Hela\r\ncell lysates probed with STAT6 monoclonal Antibody, Unconjugated (bsm-52239R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human Hela\r\ncell lysates probed with STAT6 monoclonal Antibody, Unconjugated (bsm-52239R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$25 ug total protein per lane of various lysates (see on figure) probed with STAT6 monoclonal antibody, unconjugated (bsm-52239R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with STAT6 monoclonal antibody, unconjugated (bsm-52239R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52239r-100ul
Product Name	STAT6 (9A7) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human STAT6 protein, around 700-800aa.
Clonality	Monoclonal
Clone #	9A7
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6778
Swiss Prot	P42226
Subcellular location	Cytoplasm, Nucleus
Synonyms	STAT6B; STAT6C; D12S1644; IL-4-STAT; Signal transducer and activator of transcription 6; IL-4 Stat; STAT6
Background	Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC