GAPDH Recombinant Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52262r-apc-cy7-100uL |
| Product Name | GAPDH Recombinant Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Zebrafish, Monkey |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2597 |
| Swiss Prot | P04406 |
| Synonyms | Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH |
| Background | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. |
| Application Dilution | |
| WB | 1:5000-500000 |
| FCM | FCM1ug/Test |
| IF(IHC-P) | IFIHC-P1:200-1000 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is GAPDH Recombinant Antibody, APC-Cy7 Conjugated (bsm-52262R-APC-Cy7) validated for, and at what dilution?
bsm-52262R-APC-Cy7 is validated for WB(WB) (1:5000-500000); FCM(FCM) (1ug/Test); IF(IHC-P)(IF(IHC-P)) (1:200-1000); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GAPDH Recombinant Antibody, APC-Cy7 Conjugated (bsm-52262R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Zebrafish, Monkey.
How should bsm-52262R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bsm-52262R-APC-Cy7)?
APC-Cy7 on bsm-52262R-APC-Cy7: excitation / emission 650nm/780nm.