Beta-Catenin (1A5) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52263R-100uL |
| Product Name | Beta-Catenin (1A5) Monoclonal Antibody |
| Applications |
WB
IHC
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human beta Catenin aa 1-200 |
| Clonality | Monoclonal |
| Clone # | 1A5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 1499 |
| Swiss Prot | P35222 |
| Synonyms | Catenin beta-1, Beta-catenin, Beta-catenin; CTNNB1; CTNNB |
| Background | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is Beta-Catenin (1A5) Monoclonal Antibody (bsm-52263R) validated for, and at what dilution?
bsm-52263R is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Beta-Catenin (1A5) Monoclonal Antibody (bsm-52263R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52263R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Beta-Catenin (1A5) Monoclonal Antibody (bsm-52263R)?
Human beta Catenin aa 1-200