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Histone H3 (mono methyl K36) (6G6) Monoclonal Antibody

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-52298R-100uL
Product Name Histone H3 (mono methyl K36) (6G6) Monoclonal Antibody
Applications
WB IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic peptide surrounding K36 of Histone H3
Modification Site mono methyl K36
Clonality Monoclonal
Clone # 6G6
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Swiss Prot P68431
Synonyms Histone H3.3, H3f3a
Background Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Histone H3 (mono methyl K36) (6G6) Monoclonal Antibody (bsm-52298R) validated for, and at what dilution?

bsm-52298R is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Histone H3 (mono methyl K36) (6G6) Monoclonal Antibody (bsm-52298R) react with?

Tested reactivity: Human, Mouse.

How should bsm-52298R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Histone H3 (mono methyl K36) (6G6) Monoclonal Antibody (bsm-52298R)?

Synthetic peptide surrounding K36 of Histone H3

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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