eIF-2a (Ser51) Monoclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52306r-cy55-100ul |
| Product Name | eIF-2a (Ser51) Monoclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | Synthetic phospho-peptide surrounding Ser51 of human eIF-2a |
| Modification Site | Ser51 |
| Clonality | Monoclonal |
| Clone # | 8B5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83939 |
| Swiss Prot | Q9BY44 |
| Synonyms | Eukaryotic translation initiation factor 2A, 65 kDa eukaryotic translation initiation factor 2A, EIF2A, CDA02, MSTP004, MSTP089 |
| Background | Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner. |
| Application Dilution | |
| WB | 1:100-200 |
| IF | 1:100-500 |
| Flow-Cyt | 1:500-2000 |
Frequently asked questions
Which applications is eIF-2a (Ser51) Monoclonal Antibody, Cy5.5 Conjugated (BSM-52306R-CY5-5) validated for, and at what dilution?
BSM-52306R-CY5-5 is validated for WB (1:100-200); IF (1:100-500); Flow-Cyt (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF-2a (Ser51) Monoclonal Antibody, Cy5.5 Conjugated (BSM-52306R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-52306R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF-2a (Ser51) Monoclonal Antibody, Cy5.5 Conjugated (BSM-52306R-CY5-5)?
Synthetic phospho-peptide surrounding Ser51 of human eIF-2a
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-52306R-CY5-5)?
Cy5.5 on BSM-52306R-CY5-5: excitation / emission 675nm/694nm.