CD8 alpha (72B1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52364R-100uL |
| Product Name | CD8 alpha (72B1) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant human CD8 alpha near C-terminus |
| Clonality | Monoclonal |
| Clone # | 72B1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 925 |
| Swiss Prot | P01732 |
| Subcellular location | Extracellular |
| Synonyms | T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD_antigen: CD8a, CD8A, MAL |
| Background | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is CD8 alpha (72B1) Monoclonal Antibody (bsm-52364R) validated for, and at what dilution?
bsm-52364R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD8 alpha (72B1) Monoclonal Antibody (bsm-52364R) react with?
Tested reactivity: Human.
How should bsm-52364R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD8 alpha (72B1) Monoclonal Antibody (bsm-52364R)?
Recombinant human CD8 alpha near C-terminus