14-3-3 alpha+beta (3C5) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52710R-100uL |
| Product Name | 14-3-3 alpha+beta (3C5) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide corresponding to C terminal human 14-3-3 alpha + beta |
| Clonality | Monoclonal |
| Clone # | 3C5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 7529 |
| Swiss Prot | P31946 |
| Synonyms | 14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB |
| Background | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) validated for, and at what dilution?
bsm-52710R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52710R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R)?
Synthetic peptide corresponding to C terminal human 14-3-3 alpha + beta