14-3-3 Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52728r-100uL |
| Product Name | 14-3-3 Recombinant Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein of Human 14-3-3 between 200-300 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7529 |
| Swiss Prot | P31946; P63104 |
| Synonyms | 14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB, YWHAZ |
| Background | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is 14-3-3 Recombinant Antibody (bsm-52728R) validated for, and at what dilution?
bsm-52728R is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 14-3-3 Recombinant Antibody (bsm-52728R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bsm-52728R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise 14-3-3 Recombinant Antibody (bsm-52728R)?
Recombinant protein of Human 14-3-3 between 200-300 amino acids