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Actin (6C4) Monoclonal Antibody

Applications

  • WB
  • IF(ICC)
  • IHC
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
  • Zebrafish
Overview
Catalog # bsm-52768R-100uL
Product Name Actin (6C4) Monoclonal Antibody
Applications
WB IF(ICC) IHC IHC-P
Reactivity Human, Mouse, Rat, Zebrafish
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein of human Actin corresponding to aa 50-150
Clonality Monoclonal
Clone # 6C4
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 58
Swiss Prot P68133
Synonyms Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1
Background The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200
IHC 1:50-200
IHC-P 1:200-400

Frequently asked questions

Which applications is Actin (6C4) Monoclonal Antibody (bsm-52768R) validated for, and at what dilution?

bsm-52768R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Actin (6C4) Monoclonal Antibody (bsm-52768R) react with?

Tested reactivity: Human, Mouse, Rat, Zebrafish.

How should bsm-52768R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Actin (6C4) Monoclonal Antibody (bsm-52768R)?

Recombinant protein of human Actin corresponding to aa 50-150

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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