Bcl-2 Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52833R-100uL |
| Product Name | Bcl-2 Recombinant Antibody |
| Applications |
WB
ELISA
IHC-F
IF
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Bcl-2 |
| Clonality | Recombinant |
| Clone # | 6C4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 596 |
| Swiss Prot | P10415 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Bcl-2; PPP1R50; BCL2_HUMAN; BCL2; BCL2 apoptosis regulator; B-cell CLL/lymphoma 2; BCL2, apoptosis regulator; protein phosphatase 1, regulatory subunit 50 |
| Background | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). |
| Application Dilution | |
| WB | 1:2000-10000 |
| ELISA | 1:5000-20000 |
| IHC-F | 1:500-2000 |
| IF | 1:200-1000 |
| IHC-P | 1:500-2000 |
Frequently asked questions
Which applications is Bcl-2 Recombinant Antibody (bsm-52833R) validated for, and at what dilution?
bsm-52833R is validated for WB (1:2000-10000); ELISA (1:5000-20000); IHC-F (1:500-2000); IF (1:200-1000); IHC-P (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bcl-2 Recombinant Antibody (bsm-52833R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Chicken.
How should bsm-52833R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bcl-2 Recombinant Antibody (bsm-52833R)?
KLH conjugated synthetic peptide derived from human Bcl-2