MCM5 (9G7) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54262R-100uL |
| Product Name | MCM5 (9G7) Monoclonal Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within C terminal human MCM5. |
| Clonality | Monoclonal |
| Clone # | 9G7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 4174 |
| Swiss Prot | P33992 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DNA replication licensing factor MCM5; P1-CDC46; MCM5; CDC46; minichromosome maintenance complex component 5. |
| Background | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is MCM5 (9G7) Monoclonal Antibody (bsm-54262R) validated for, and at what dilution?
bsm-54262R is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MCM5 (9G7) Monoclonal Antibody (bsm-54262R) react with?
Tested reactivity: Human, Mouse.
How should bsm-54262R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MCM5 (9G7) Monoclonal Antibody (bsm-54262R)?
Recombinant protein within C terminal human MCM5.