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Aconitase 2 (2F4) Monoclonal Antibody

Applications

  • WB
  • IF(IHC-P)
  • IF(ICC)
  • IP
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-54442R-100uL
Product Name Aconitase 2 (2F4) Monoclonal Antibody
Applications
WB IF(IHC-P) IF(ICC) IP IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within human Aconitase 2 aa 1-150.
Clonality Monoclonal
Clone # 2F4
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 50
Swiss Prot Q99798
Subcellular location Mitochondrion
Synonyms Aconitate hydratase mitochondrial; Citrate hydro-lyase; Aconitase; ACO2
Background Aconitase 2, mitochondrial is a protein that in humans is encoded by the ACO2 gene. The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15 (PRSS15), also known as Lon protease, after oxidative modification.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IP 1-2ug
IHC-P 1:200-400

Frequently asked questions

Which applications is Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) validated for, and at what dilution?

bsm-54442R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IP (1-2ug); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-54442R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R)?

Recombinant protein within human Aconitase 2 aa 1-150.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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