Histone H3 (mono methyl R2) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54537R-100uL |
| Product Name | Histone H3 (mono methyl R2) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein |
| Modification Site | Arg2 |
| Clonality | Monoclonal |
| Clone # | 2C3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 8290 |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus, Chromosome |
| Synonyms | HIST1 cluster, H3J antibody, Histone gene cluster 1, H3 histone family, member E antibody, Histone gene cluster 1, H3G antibody, H3 histone family, member J antibody, HIST1 cluster, H3E antibody, HIST1 cluster, H3I antibody, Histone gene cluster 1, H3C antibody, FLJ92264 antibody, H3 histone family, member A antibody |
| Background | Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Histone H3 (mono methyl R2) Monoclonal Antibody (bsm-54537R) validated for, and at what dilution?
bsm-54537R is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (mono methyl R2) Monoclonal Antibody (bsm-54537R) react with?
Tested reactivity: Human, Mouse.
How should bsm-54537R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (mono methyl R2) Monoclonal Antibody (bsm-54537R)?
Recombinant protein