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CD45 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD45 Monoclonal Antibody, Unconjugated (bsm-54757R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD45 Monoclonal Antibody, Unconjugated (bsm-54757R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD45 Monoclonal Antibody, Unconjugated (bsm-54757R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD45 Monoclonal Antibody, Unconjugated (bsm-54757R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD45 Monoclonal Antibody, Unconjugated (bsm-54757R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD45 Monoclonal Antibody, Unconjugated (bsm-54757R) at 1:200 for 30 minutes at room temperature, DAB staining.
Jurkat cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD45 Monoclonal Antibody(bsm-54757R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).
Jurkat cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD45 Monoclonal Antibody(bsm-54757R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).

Applications

  • WB
  • FCM
  • IHC-P
  • IHC

Reactivity

  • Human
Overview
Catalog # bsm-54757R
Product Name CD45 Monoclonal Antibody
Applications WB, FCM, IHC-P, IHC
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic Peptide corresponding to the C-terminal human CD45.
Clonality Monoclonal
Clone # 5H16
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 5788
Swiss Prot P08575
Subcellular location Cell membrane, Cell junction, Membrane
Synonyms B220 antibody, CD 45 antibody, CD45 antibody, CD45 antigen antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, Leukocyte common antigen antibody, loc antibody, Ly-5 antibody, LY5 antibody, Ly5, homolog of antibody, Lyt-4 antibody, OTTHUMP00000033813 antibody, OTTHUMP00000033816 antibody, OTTHUMP00000033817 antibody, OTTHUMP00000038574 antibody, Protein tyrosine phosphatase receptor type c polypeptide antibody, Protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, Protein tyrosine phosphatase, receptor type, c polypeptide antibody, Ptprc antibody, PTPRC_HUMAN antibody, Receptor-type tyrosine-protein phosphatase C antibody, T200 antibody, T200 glycoprotein antibody, T200 leukocyte common antigen antibody
Background The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC

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