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Acetyl-Histone H3 (Lys4) Monoclonal Antibody

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:400\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Pharbitis\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:400\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Pharbitis\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Bioss unconjugated antibody vial

Applications

  • WB
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60057m-100uL
Product Name Acetyl-Histone H3 (Lys4) Monoclonal Antibody
Applications
WB ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Clonality Monoclonal
Clone # H4G1
Isotype IgG
Concentration Lot Dependent
Purification Protein G coupled with the antigen peptide containing acetylated H3 (Lys4).
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P68431
Application Dilution
WB =1:400-1:2000
ICC/IF ICC/IF=1:50-1:200

Frequently asked questions

Which applications is Acetyl-Histone H3 (Lys4) Monoclonal Antibody (bsm-60057M) validated for, and at what dilution?

bsm-60057M is validated for WB (WB=1:400-1:2000); ICC/IF (ICC/IF=1:50-1:200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Acetyl-Histone H3 (Lys4) Monoclonal Antibody (bsm-60057M) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-60057M be stored?

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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