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Tri-Methyl-Histone H3 (Lys27) Monoclonal Antibody

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin-fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60136M
Tissue: Human neuroblastoma\r\nSection type: Formalin-fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60136M
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
  • Monkey
Overview
Catalog # bsm-60136m-100uL
Product Name Tri-Methyl-Histone H3 (Lys27) Monoclonal Antibody
Applications
WB IHC-P IHC-F IF ICC/IF
Reactivity Human, Mouse, Rat, Monkey
Specifications
Conjugation Unconjugated
Host Mouse
Clonality Monoclonal
Clone # B11C10
Isotype IgG
Concentration Lot Dependent
Purification Protein G coupled with the antigen peptide containing H3K27me3.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P68431
Application Dilution
WB =1:500-1:2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=0
ICC/IF ICC/IF=1:50-1:100

Frequently asked questions

Which applications is Tri-Methyl-Histone H3 (Lys27) Monoclonal Antibody (bsm-60136M) validated for, and at what dilution?

bsm-60136M is validated for WB (WB=1:500-1:2000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=0); ICC/IF (ICC/IF=1:50-1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Tri-Methyl-Histone H3 (Lys27) Monoclonal Antibody (bsm-60136M) react with?

Tested reactivity: Human, Mouse, Rat, Monkey.

How should bsm-60136M be stored?

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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