Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

ADAM10 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAM10 Monoclonal Antibody, Unconjugated (bsm-60407M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAM10 Monoclonal Antibody, Unconjugated (bsm-60407M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Right occipital lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAM10 Monoclonal Antibody, Unconjugated (bsm-60407M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Right occipital lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAM10 Monoclonal Antibody, Unconjugated (bsm-60407M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
The Jurkat (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-ADAM10 antibody (bsm-60407M,1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-ADAM10 antibody (bsm-60407M,1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (ADAM10) monoclonal Antibody, unconjugated (bsm-60407M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (ADAM10) monoclonal Antibody, unconjugated (bsm-60407M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF(IHC-P)
  • IF(ICC)
  • IHC-P
  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-60407m-100uL
Product Name ADAM10 Monoclonal Antibody
Applications
IHC-F IF(IHC-P) IF(ICC) IHC-P FCM
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Clonality Monoclonal
Clone # H1D3
Isotype IgG1/kappa
Concentration Lot Dependent
Purification Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot O14672
Application Dilution
IHC-F 1:50-200
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IHC-P 1:50-200
FCM 10ul/10^6 cells in 100ul

Frequently asked questions

Which applications is ADAM10 Monoclonal Antibody (bsm-60407M) validated for, and at what dilution?

bsm-60407M is validated for IHC-F (1:50-200); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:50-200); FCM (10ul/10^6 cells in 100ul). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADAM10 Monoclonal Antibody (bsm-60407M) react with?

Tested reactivity: Human.

How should bsm-60407M be stored?

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Search our catalog of hundreds of thousands of products

Free sample offer

Try a recombinant sample

20 µL sample

Freewith your paid order

View product details (opens in a new tab)

View cart

US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

Primary antibody added to your cart

Need a secondary antibody?

Product validation image

View product details and validation

Confirm application and cross-reactivity before use. Prices shown are before eligible cart discounts. Shipping and tax are calculated at checkout.

View cart