CD80 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60904m-100uL |
| Product Name | CD80 Monoclonal Antibody |
| Applications |
IHC-F
IF(IHC-P)
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human CD80 |
| Clonality | Monoclonal |
| Clone # | 13A5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 941 |
| Swiss Prot | P33681 |
| Subcellular location | Cell membrane |
| Synonyms | Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80. |
| Background | CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. |
| Application Dilution | |
| IHC-F | 1:50-200 |
| IF(IHC-P) | 1:50-200 |
| IHC-P | 1:50-200 |
Frequently asked questions
Which applications is CD80 Monoclonal Antibody (bsm-60904M) validated for, and at what dilution?
bsm-60904M is validated for IHC-F (1:50-200); IF(IHC-P) (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD80 Monoclonal Antibody (bsm-60904M) react with?
Tested reactivity: Human.
How should bsm-60904M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD80 Monoclonal Antibody (bsm-60904M)?
KLH conjugated synthetic peptide derived from human CD80