Gamma Tubulin Recombinant Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61186r-fitc-100uL |
| Product Name | Gamma Tubulin Recombinant Antibody, FITC Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Chicken, Zebrafish, Monkey, Hamster, Xenopus |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human gamma 1 Tubulin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7283 |
| Swiss Prot | P23258 |
| Subcellular location | Cytoplasm |
| Synonyms | Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1; TBG1; TUBG; TUBG1; TUBGCP1; Tubulin gamma-1 chain; tubulin, gamma 1; tubulin, gamma polypeptide,gamma tubulin. |
| Background | Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. |
| Application Dilution | |
| WB | 1:50-100 |
| IF | 1:100-500 |
| Flow-Cyt | 1:500-2000 |
Frequently asked questions
Which applications is Gamma Tubulin Recombinant Antibody, FITC Conjugated (bsm-61186R-FITC) validated for, and at what dilution?
bsm-61186R-FITC is validated for WB (1:50-100); IF (1:100-500); Flow-Cyt (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Gamma Tubulin Recombinant Antibody, FITC Conjugated (bsm-61186R-FITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Chicken, Zebrafish, Monkey, Hamster, Xenopus.
How should bsm-61186R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Gamma Tubulin Recombinant Antibody, FITC Conjugated (bsm-61186R-FITC)?
KLH conjugated synthetic peptide derived from human gamma 1 Tubulin
What are the excitation and emission wavelengths of the FITC conjugate (bsm-61186R-FITC)?
FITC on bsm-61186R-FITC: excitation / emission 494nm/518nm.
