ATG4A Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61617r-cy7-100uL |
| Product Name | ATG4A Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cysteine protease ATG4A |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 115201 |
| Swiss Prot | Q8WYN0 |
| Subcellular location | Cytoplasm |
| Synonyms | Apg4a; ATG4A; Atg4al; AUT like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2; Autophagy related 4A cysteine peptidase. |
| Background | Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is ATG4A Recombinant Antibody, Cy7 Conjugated (bsm-61617R-Cy7) validated for, and at what dilution?
bsm-61617R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATG4A Recombinant Antibody, Cy7 Conjugated (bsm-61617R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61617R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATG4A Recombinant Antibody, Cy7 Conjugated (bsm-61617R-Cy7)?
KLH conjugated synthetic peptide derived from human Cysteine protease ATG4A
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-61617R-Cy7)?
Cy7 on bsm-61617R-Cy7: excitation / emission 743nm/767nm.