Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62431r-100uL |
| Product Name | Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Antibody |
| Applications |
WB
ICC/IF
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histone H3.1 around the methylation site of K15 |
| Modification Site | Lys15 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 8350 |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | H3 histone; HIST1H3A; Histone cluster 1, H3a;H3/l; HIST3H3; H3K14me1; H3K14me2; H3K14me3. |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Antibody (bsm-62431R) validated for, and at what dilution?
bsm-62431R is validated for WB (1:300-5000); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Antibody (bsm-62431R) react with?
Tested reactivity: Human, Mouse.
How should bsm-62431R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Antibody (bsm-62431R)?
KLH conjugated synthetic peptide derived from human Histone H3.1 around the methylation site of K15