c-Jun & JunD (phospho-S73/S100) Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining\u3002$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining\u3002

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining\u3002$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining\u3002

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining\u3002$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73\/S100) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining\u3002

$NIH\/3T3 (M) cells were treated with or without anisomycin (25\u03bcg\/mL) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-c-Jun (Thr91+Thr93) monoclonal antibody, unconjugated (bsm-62758R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

NIH\/3T3 (M) cells were treated with or without anisomycin (25\u03bcg\/mL) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-c-Jun (Thr91+Thr93) monoclonal antibody, unconjugated (bsm-62758R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IP
IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-62758r-100ul
Product Name	c-Jun & JunD (phospho-S73/S100) Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human c Jun around the phosphorylation site of S73
Modification Site	Ser73+Ser100
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3725
Swiss Prot	P05412,P17535
Subcellular location	Nucleus
Synonyms	JunD; c-Jun.
Background	Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF